Custom Writing Service-Flow Cytometry
HeLa cells were treated with a new experimental drug (thought to inhibit BCL-2) to rule out any potential cytotoxic effects. Cells were exposed to the drug for 24 hours prior to harvesting (B) or a vehicle control (A). The cells were then harvested and stained with Annexin V – FITC and propidium iodide (PI) before analysis by flow cytometry. Fluorescence due to FITC is measured by the FL1-H channel and fluorescence due to PI is measured by the FL3-H channel. (Refer to Screen Shot 2018-07-20 at 7.51.50 AM)
Annexin V can bind to phosphatidylserine. What does an increase in Annexin V binding represent?
What percentage of cells are viable in the vehicle control?
What effect does the drug have on HeLa cells?
What effect might over-expressing BCL-2 in the HeLa cells prior exposure to the drug have?
