BL2214 Assignment Essay help-The effect of metabolites on myosin ATPase (muscle contraction) as a possible mechanism of peripheral muscle fatigue
BL2214 Assignment 2 – Illustrated Abstract
The effect of metabolites on myosin ATPase (muscle contraction) as a possible mechanism of peripheral muscle fatigue
It is well established that contraction of striated muscle is due to the cyclic interaction of myosin in the thick filaments of the sarcomere and actin in the thin filaments. Myosin heads attach to actin and the resultant structural change leads to filament sliding and the subsequent detachment of the heads. This is thought to occur asynchronously many times during contraction. Adenosine triphosphate (ATP) provides the immediate source of energy for muscle contraction. Hydrolysis of the bonds between the phosphate residues of ATP are associated with the release of a large amount of energy. In muscle the hydrolysis of ATP to adenosine diphosphate (ADP) is catalysed by the contractile protein myosin, thus adenosine triphosphatase (ATPase) activity is found in the heads of the myosin molecules where they are in contact with actin.
In these studies use is made of glycerinated fibres of rabbit psoas muscle, which provides an excellent opportunity for practical studies of muscle ultrastructure and mechanism of contraction. A Glycerinated muscle fibre is obtained by taking muscle fibres from a freshly killed animal and immersing in equal volumes of water and glycerol at 00C and subsequently storing at -200C. This treatment leaves the contractile structure intact whilst perforating the cell membrane (sarcolemma) and removing other factors such as ATP and destroying the ATP-restoring systems. By the use of appropriate chemicals, the fibre can be made to relax, to contract or be made extensible.
Assignment
Submit an Illustrated Abstract written and styled using standard scientific writing guidelines.
Data for the assignment will be based on the class data from week 2 of the muscle labs.
Word count 500 words ±10%
The assignment accounts for 40% of the overall module marks.
The indicative timetable for the assessed components is shown below.
| Assignment | Date set | Deadline | Feedback date |
| Practical abstract | 8th March 2018 | 26th April 2018 2pm | 31st May 2018 |
On following page is an outline of what you should include/consider.
If you have any questions with respect to this work, please ask!!!!!
Below are suggested optional subheadings for the illustrated abstract and further guidance about the content of each.
Introduction, aims and hypotheses.
This should provide a brief review of literature including a discussion of our current knowledge of the mechanisms of peripheral muscle fatigue specifically at the myosin head and involving myosin ATPase.
The introduction should finish with clearly defined aims of the study and any hypotheses you may have had before collecting data.
You must cite the original journal articles.
Methods.
This should be written in the past tense and your own words referring to the laboratory handout to keep the word count down. Avoid using bulleted lists (look at the Journal of Physiology as a guide for formatting methods sections in Research papers).
https://jp.msubmit.net/cgi-bin/main.plex?form_type=display_requirements
It is not necessary to include a list of equipment but instead to give a clear indication of the protocol used, identifying the control and the experimental conditions. Make reference to the statistical analysis.
Results.
Results should be presented using appropriate graphs. For each variable plotted, i.e.pH and [Pi], make sure you include a brief commentary for the results and any statistical data. Present the means and standard deviations from the class data. The figures including the legend are excluded from the word count.
Describe the statistical test used on the data. Use an appendix for the SPSS output table This is excluded from the word count
Discussion.
Clear evidence of critical evaluation of the results, rather than simply describing what happened. This section should discuss effect of H+ and Pi on contraction and whether these physiological variables may have an impact on a human subject. How do results compare with those previously reported in the literature? Explain the reason for any differences that you have shown.
Conclusions.
Link the results to the aim of the experiment, accept or refute any hypotheses previously stated. State what can be concluded from the analysis and discussion of the data collected in this investigation. Suggest further refinement of the work.
Excluded from the word count.
References.
A complete reference list using the Harvard referencing system for each of the citations in the text of the illustrated abstract.
Appendices:
Put your SPSS output for the statistical tests here.
Darrell
January 2018
